• Cyrano linc oip I Rik is a long intergenic

  2018-11-08

  

  Cyrano (linc-oip5, 1700020I14Rik) is a long intergenic ncRNA (lincRNA) transcribed in mouse ESCs (Chew et al., 2013; Guttman et al., 2010; Ulitsky et al., 2011) that was first characterized in zebrafish (Ulitsky et al., 2011). In zebrafish, it is a key regulator that functions in brain, eye, and nas

• In this study hPSC derived

  2018-11-08

  

  In this study hPSC-derived bfCNs were generated via an expandable NSC intermediate, which maintained the desired basal forebrain progenitor phenotype, and neurogenic capacity even after long-term expansion in culture. This scalability is a required feature of any cell type to be used in regenerative

• DE cells were recently generated from mouse

  2018-11-08

  

  DE OG-L002 manufacturer were recently generated from mouse embryoid bodies using a combination of ActA (TGFβ activator), Noggin (BMP antagonist), and lithium chloride (Wnt pathway activator) (Li et al., 2011). Whereas ActA and Wnt activators are commonly used for DE induction, Noggin supplementatio

• Finally we provide molecular evidence for PB transposase act

  2018-11-08

  

  Finally, we provide molecular evidence for PB transposase activity in vivo after plasmid intramuscular injection and electrotransfer of the murine muscles, for instance from the recovery and sequencing of several transposon–genomic junctions. Interestingly, such integration events were not able to m

• br Cardiac niches and the

  2018-11-08

  

  Cardiac niches and the Notch receptor system The Notch pathway is an evolutionary conserved intercellular and intracellular mechanism that controls stem cell fate (Bray, 2006). The Notch gene family encodes transmembrane receptors that are activated by ligand binding and proteolytic cleavage, res

• Our results are in line with

  2018-11-08

  

  Our results are in line with previous findings showing that cellular senescence negatively correlates with reprogramming efficiency (Banito et al., 2009; Mali et al., 2008; Qin et al., 2007; Zhao and Daley, 2008; Zhao et al., 2008). The expression of p21 is controlled by p53 during the replicative s

• Mesenchymal stem cells MSCs can be isolated from various

  2018-11-08

  

  Mesenchymal stem trp channel (MSCs) can be isolated from various adult tissues (including bone marrow), they are multipotent and can differentiate into cells of the mesodermal (e.g. adipocytes, osteocytes, chondrocytes) as well as other embryonic lineages (Jiang et al., 2002). MSCs can also be cult

• Creating a genetically diverse set of differentiated cells

  2018-11-08

  

  Creating a genetically diverse set of differentiated fda approved for the purposes of phenotype-genotype studies would have great value in pharmacogenomics, a field that has relied primarily on EBV-transformed lymphoblastoid cell lines (Moen et al., 2012; Wheeler and Dolan, 2012; Welsh et al., 2009)

• Fortunately a new class of RNA guided

  2018-11-08

  

  Fortunately, a new class of RNA-guided nucleases has been developed from microbial adaptive immune systems (i.e. clustered regularly interspaced short palindromic repeat-CRISPR- and CRISPR associated Cas9-CRISPR/Cas9-systems) (Cong et al., 2013; Mali et al., 2013a). This system provides a very simpl

• br Materials and methods br

  2018-11-08

  

  Materials and methods Acknowledgements Mef2c-AHF-Cre mice were generously provided by Dr. Brian L. Black. We thank Mamen Martín and Sandra Rodríguez (Molecular Cytogenetics Unit, CNIO) for technical advice; and the Imaging Core Facility for the confocal analysis (CIMA, University of Navarra).

• A number of animal models of CLI including the hindlimb

  2018-11-08

  

  A number of animal models of CLI, including the hindlimb ischemia model after femoral artery ligation in rats (Rochester et al., 1994) and rabbits (Hao et al., 2014), have been proven useful to assess the effects of various cell types and to study potential mechanisms of action. For instance, IM inj

• Using bisulfite sequencing and Southern blotting

  2018-11-08

  

  Using bisulfite sequencing and Southern blotting analyses, we demonstrated previously that the major and minor satellites of pericentromeric heterochromatin are hypomethylated only in germ cell lineages, whereas all somatic guanylyl cyclase are hypermethylated, and that this DNA methylation profile

• Treatment options for xerostomia include administration of s

  2018-11-08

  

  Treatment options for xerostomia include administration of saliva substitutes or stimulants (Fox, 2004). Saliva substitutes might improve some, but not all, problems associated with SG dysfunction, whereas stimulants are only useful for people with some remaining SG function. Alternative approaches

• Compared to treatments with other FGF isoforms stimulation w

  2018-11-08

  

  Compared to treatments with other FGF2 isoforms, stimulation with 22.5kDa FGF2 results in lower stem cell gene expression in hESCs (Fig. 4d), weaker proliferative activity in human dermal fibroblasts (Fig. 5), and lower relative mitogenic activity in BaF3 ret proto oncogene ectopically expressing FG

• br Acknowledgements This work was

  2018-11-08

  

  Acknowledgements This work was supported by the IRET Foundation (2015-2016), Ozzano Emilia, Italy (no-profit organization), in the frame of the “Step-by-step” POR-FESR 2016-2020 Emilia Romagna Region project. Introduction Resident neural stem cells (NSCs) persist in the adult mammalian centra

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